Developed by Kinya Otsu, Osaka University Graduate School of Medicine in 2000. The targeting construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J. true Cell Biology Research Mitogen-activated protein (MAP) kinase cascades are highly conserved signal transduction pathways involved in a variety of physiological events. The MAP kinase family consists of extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal protein kinase (JNK), and p38 MAP kinase. p38 alpha, one of the p38 MAP kinase subfamily, is widely expressed and has an important function in cytokine production and the response to many types of stress. Because p38alpha-deficient mice (BRC00361 C57BL/6-TgH(p38)/118) showed embryonic lethality at midgestation, this strain is useful for the research of p38 MAP kinase pathway in various tissues using Cre-loxP system. <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/jul_2007_mm" target="_blank">Mouse of the Month Jul 2007</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/02192_GB.pdf">Genetic Background</a> Phage P1 loxP sites, mouse p38 alpha genomic DNA 大津　欣也 R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile. p38α-flox mouse C (3-6 months) 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Mol. Cell. Biol., 24, 10611-10620 (2004).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請及び事業活動を行う場合は、寄託者と別途協議を行う。 RBRC02192 Osaka Univ. p38αfloxマウスは、p38αfloxATP結合部位を含むエクソンの両端イントロン部分にloxP配列を導入。Creリコンビナーゼ発現細胞でp38αをノックアウトできる。 p38α-flox マウス The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell. Biol., 24, 10611-10620 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 国立大学法人大阪大学 Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="http://www.cardiology.med.osaka-u.ac.jp/" target="_blank">Lab HP (Japanese)</A> C57BL6背景のホモp38αfloxマウス同士の交配で系統を維持している。 C（3〜6か月） B6.129-Mapk14<tm1.2Otsu> B6.129-Mapk14<tm1.2Otsu> General Purpose <a href='https://brc.riken.jp/mus/pcr02192'>Genotyping protocol -PCR-</a> Cre/loxP system Kinya OTSU p38αコンベンショナルノックアウトマウスが胎生致死であるため、生体でのp38αの機能を評価する目的で開発した。開発年：2000年　開発者：大津欣也先生　機関名：大阪大学大学院医学系研究科